GK092 ScienCell 人胰腺星狀細(xì)胞生物學(xué)qPCR檢測試劑盒，Human Pancreatic Stellate Cell Biology qPCR Array Kit

ScienCell 研究實驗室（ScienCell）是一家生物技術(shù)公司，其使命是實驗和治療用細(xì)胞和細(xì)胞相關(guān)產(chǎn)品的研究和發(fā)展。 ScienCell為研究界提供各種高質(zhì)量的正常的人類和動物細(xì)胞，細(xì)胞培養(yǎng)基和試劑，培養(yǎng)基添加因子，細(xì)胞來源的RNA，cDNA和蛋白質(zhì)。ScienCell的科學(xué)家們以改善生活質(zhì)量，實現(xiàn)細(xì)胞的能力，打擊人類退化性疾病為目的研究和開發(fā)各種細(xì)胞治療策略。ScienCell總部設(shè)在美國加利福尼亞州圣地亞哥，公司成立于1999年。

北京諾博萊德作為ScienCell品牌的經(jīng)銷商，為客戶提供，正規(guī)進(jìn)口，貨期穩(wěn)定，折扣好的服務(wù)標(biāo)準(zhǔn)。

GK092 ScienCell人胰腺星狀細(xì)胞生物學(xué)qPCR檢測試劑盒，Human Pancreatic Stellate Cell Biology qPCR Array Kit，產(chǎn)品簡介：

產(chǎn)品品牌： ScienCell

產(chǎn)品貨號：GK092

產(chǎn)品名稱：GK092 ScienCell人胰腺星狀細(xì)胞生物學(xué)qPCR檢測試劑盒，Human Pancreatic Stellate Cell Biology qPCR Array Kit

產(chǎn)品規(guī)格：One 96-well plate, one 20ul reaction per well

GK092 ScienCell人胰腺星狀細(xì)胞生物學(xué)qPCR檢測試劑盒，Human Pancreatic Stellate Cell Biology qPCR Array Kit， 產(chǎn)品說明：

ScienCell 的 GeneQuery™ 人類胰腺星狀細(xì)胞生物學(xué) qPCR 陣列試劑盒 （GQH-PSB） 旨在促進(jìn)參與 （i） 人胰腺星狀細(xì)胞活化的 88 個關(guān)鍵基因的基因表達(dá)譜;（二）視黃醇代謝;（iii）細(xì)胞外基質(zhì)（ECM）合成和重塑;以及（iv）胰腺星狀細(xì)胞相關(guān)疾病。如何根據(jù)其功能對所包含的基因進(jìn)行分組的簡要示例如下所示：  

胰腺星狀細(xì)胞標(biāo)志物：ACTA2，GFAP，COL11A1，DES，NGFR，POSTN，ITGA7，CTGF

胰腺星狀細(xì)胞活化：ICAM1，MMP13，RELN，TIMP1，SYP，CDH1，CDH2，RARA，PDGF，TGFB1，MMP2

視黃醇代謝： RBP1， RBP2， RBP4， RBP5， CRABP1， CRABP2， LRAT， CYP26s

ECM合成和重塑：TGFB1，COL4A2，層粘連蛋白亞基，SPARC，VCAN，CTGF

細(xì)胞因子生產(chǎn)：IL1A，IL1B，IL6，PDGF，TGFBs，TNF

p53 信號通路： CCNB1， CDK1， CCNE1， RRM2， GADD45G， CDK6， CHEK1， CDKN1A， EI24， ZMAT3

胰腺纖維化和癌癥發(fā)展：ACVR1B，CCKBR，CDKN2A，CELSR3，EGFR，GNL3，PALLD，RBBP8，RIMS1，SMAD4，STK11

GeneQuery™ qPCR 芯片試劑盒采用 96 孔板格式的 qPCR 就緒，每個孔包含一個引物組，可以特異性識別并有效擴(kuò)增靶基因的 cDNA。精心設(shè)計的引物確保：（i）qPCR分析中的優(yōu)良退火溫度為65°C（含2 mM Mg2+，不含DMSO）;（ii）除非另有說明，否則引物組可識別靶基因的所有已知轉(zhuǎn)錄本變體;（iii）只有一個基因被擴(kuò)增。每個引物組都已通過qPCR和熔解曲線分析和凝膠電泳進(jìn)行了驗證。

ScienCell's GeneQuery™ Human Pancreatic Stellate Cell Biology qPCR Array Kit (GQH-PSB) is designed to facilitate gene expression profiling of 88 key genes involved in (i) human pancreatic stellate cell activation; (ii) retinol metabolism; (iii) extracellular matrix (ECM) synthesis and remodeling; and (iv) pancreatic stellate cell related disorders. Brief examples of how included genes may be grouped according to their functions are shown below:    

·         Pancreatic stellate cell markers: ACTA2, GFAP, COL11A1, DES, NGFR, POSTN, ITGA7, CTGF

·         Pancreatic stellate cell activation: ICAM1, MMP13, RELN, TIMP1, SYP, CDH1, CDH2, RARA, PDGFs, TGFB1, MMP2

·         Retinol metabolism: RBP1, RBP2, RBP4, RBP5, CRABP1, CRABP2, LRAT, CYP26s

·         ECM synthesis and remodeling: TGFB1, COL4A2, Laminin subunits, SPARC, VCAN, CTGF

·         Cytokine production: IL1A, IL1B, IL6, PDGFs, TGFBs, TNF

·         p53 signaling pathway: CCNB1, CDK1, CCNE1, RRM2, GADD45G, CDK6, CHEK1, CDKN1A, EI24, ZMAT3

·         Pancreatic fibrosis and cancer development: ACVR1B, CCKBR, CDKN2A, CELSR3, EGFR, GNL3, PALLD, RBBP8, RIMS1, SMAD4, STK11

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.

基因查詢™人胰腺星狀細(xì)胞生物學(xué)qPCR陣列板布局*（8 個粗體和斜體控件）

提示：基因選擇可能會根據(jù)新的研究和開發(fā)進(jìn)行更新

ScienCell基因表達(dá)分析產(chǎn)品訂購信息：

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GK020   人B細(xì)胞受體信號通路qPCR檢測試劑盒，Human B Cell Receptor Signaling Pathway qPCR Array Kit

GK021   人類T細(xì)胞受體信號通路qPCR檢測試劑盒，Human T Cell Receptor Signaling Pathway qPCR Array Kit

GK022   人內(nèi)吞作用qPCR檢測試劑盒，Human Endocytosis qPCR Array Kit

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GK026   人類Wnt信號通路qPCR檢測試劑盒，Human Wnt Signaling Pathway qPCR Array Kit

GK027   人類非經(jīng)典Wnt信號通路qPCR檢測試劑盒，Human Noncanonical Wnt Signaling Pathways qPCR Array Kit

GK028   人類Wnt信號靶基因qPCR檢測試劑盒，Human Wnt Signaling Target Genes qPCR Array Kit

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GK093   Human Renal Mesangial Cell Biology qPCR Array Kit，Human Renal Mesangial Cell Biology qPCR Array Kit

GK094   Human Mesenchymal Stem Cell Differentiation qPCR Array，Human Mesenchymal Stem Cell Differentiation qPCR Array Kit

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